Human fecal source identification with real-time quantitative PCR.

نویسندگان

  • Orin C Shanks
  • Lindsay Peed
  • Mano Sivaganesan
  • Richard A Haugland
  • Eunice C Chern
چکیده

Waterborne diseases represent a significant public health risk worldwide and can originate from contact with water contaminated with human fecal material. We describe a real-time quantitative PCR (qPCR) method that targets a genetic marker of the human-associated Bacteroides dorei for identification of human fecal pollution in ambient water samples. The following protocol includes water sample collection, filtration, DNA isolation with a sample processing control, qPCR amplification with an internal amplification control, and quality control data analysis.

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عنوان ژورنال:
  • Methods in molecular biology

دوره 1096  شماره 

صفحات  -

تاریخ انتشار 2014